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pmal c2 expression vector  (New England Biolabs)


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    Structured Review

    New England Biolabs pmal c2 expression vector
    Pmal C2 Expression Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 3348 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmal+c2+expression+vector/pMAL/chem_rxiv___2021___1whqp-46-27-30
    Average 96 stars, based on 3348 article reviews
    pmal c2 expression vector - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Construct:

    Article Title: Food phytochemicals, epigallocatechin gallate and myricetin, covalently bind to the active site of the coronavirus main protease in vitro .
    Article Snippet: Amylose resin nd Factor Xa were obtained from New England Biolabs Japan Inc. Tokyo, Japan). .. Other chemicals used were of high-quality grades, nless otherwise indicated. reparation of maltose-binding protein (MBP)-tagged main protease and its BP-cleaved protease The MBP-tagged main protease containing SARS-CoV-2 M pro (Genank Accession No. YP_009725301; Met1–Gln306) was constructed us- ng pMAL-c2 expression vector (New England Biolabs Japan Inc.). .. The ene encoding SARS-CoV-2 M pro with E. coli codon usage was syntheized by Eurofins Genomics (Tokyo, Japan).

    Article Title: Food phytochemicals, epigallocatechin gallate and myricetin, covalently bind to the active site of the coronavirus main protease in vitro
    Article Snippet: .. The MBP-tagged main protease containing SARS-CoV-2 M pro (GenBank Accession No. YP_009725301; Met1–Gln306) was constructed using pMAL-c2 expression vector (New England Biolabs Japan Inc.). .. The gene encoding SARS-CoV-2 M pro with E. coli codon usage was synthesized by Eurofins Genomics (Tokyo, Japan).

    Article Title: Food phytochemicals, epigallocatechin gallate and myricetin, covalently bind to the active site of the coronavirus main protease in vitro
    Article Snippet: .. Preparation of maltose-binding protein (MBP)-tagged main protease and its MBPcleaved protease The MBP-tagged main protease containing SARS-CoV-2 Mpro (GenBank Accession No. 105 YP_009725301; Met1–Gln306) was constructed using pMAL-c2 expression vector (New England Biolabs Japan Inc.). .. The gene encoding SARS-CoV-2 Mpro with E. coli codon usage was synthesized by Eurofins Genomics (Tokyo, Japan).

    Expressing:

    Article Title: Food phytochemicals, epigallocatechin gallate and myricetin, covalently bind to the active site of the coronavirus main protease in vitro .
    Article Snippet: Amylose resin nd Factor Xa were obtained from New England Biolabs Japan Inc. Tokyo, Japan). .. Other chemicals used were of high-quality grades, nless otherwise indicated. reparation of maltose-binding protein (MBP)-tagged main protease and its BP-cleaved protease The MBP-tagged main protease containing SARS-CoV-2 M pro (Genank Accession No. YP_009725301; Met1–Gln306) was constructed us- ng pMAL-c2 expression vector (New England Biolabs Japan Inc.). .. The ene encoding SARS-CoV-2 M pro with E. coli codon usage was syntheized by Eurofins Genomics (Tokyo, Japan).

    Article Title: Polo regulates Spindly to prevent premature stabilization of kinetochore–microtubule attachments
    Article Snippet: The recombinant proteins were purified using the Ni‐NTA purification system (Qiagen) and eluted with 250 mM Imidazole, 300 mM NaCl, 50 mM NaH 2 PO 4 , pH 8.0. .. For MBP fusion, PCR products harbouring Zwilch or Spindly coding sequences were cloned into EcoRI/XbaI sites of pMal‐c2 expression vector (New England Biolabs). ..

    Article Title: Food phytochemicals, epigallocatechin gallate and myricetin, covalently bind to the active site of the coronavirus main protease in vitro
    Article Snippet: .. The MBP-tagged main protease containing SARS-CoV-2 M pro (GenBank Accession No. YP_009725301; Met1–Gln306) was constructed using pMAL-c2 expression vector (New England Biolabs Japan Inc.). .. The gene encoding SARS-CoV-2 M pro with E. coli codon usage was synthesized by Eurofins Genomics (Tokyo, Japan).

    Article Title: Epitope mapping of an anti-diacylglycerol kinase delta monoclonal antibody DdMab-1
    Article Snippet: .. Synthesized DNA (Eurofins Genomics KK, Tokyo, Japan) encoding hDGKδ (accession No. NM_152879 ) plus a C-terminal PA tag (GVAMPGAEDDVV) [ ] was subcloned into the pMAL-c2 expression vector (New England Biolabs Inc., Beverly, MA) using the In-Fusion HD Cloning Kit (Takara Bio, Inc., Shiga, Japan). ..

    Article Title: The Membrane-Bound Notch Regulator Mnr Supports Notch Cleavage and Signaling Activity in Drosophila melanogaster
    Article Snippet: .. To this end, the coding sequence was PCR amplified, adding Eco RI and Sal I site, and inserted into pMal-c2 expression vector (NEB, Ipswich, MA, USA) in frame with N-terminal malE to generate pMal-Mnr. ..

    Article Title: Early antibody response against Mycobacterium avium subspecies paratuberculosis antigens in subclinical cattle
    Article Snippet: .. There are 92 total M. paratuberculosis proteins recombinantly produced in E. coli with 89 of these using the pMAL-c2 expression vector (New England Biolabs), and 3 using the pET vector. ..

    Article Title: Food phytochemicals, epigallocatechin gallate and myricetin, covalently bind to the active site of the coronavirus main protease in vitro
    Article Snippet: .. Preparation of maltose-binding protein (MBP)-tagged main protease and its MBPcleaved protease The MBP-tagged main protease containing SARS-CoV-2 Mpro (GenBank Accession No. 105 YP_009725301; Met1–Gln306) was constructed using pMAL-c2 expression vector (New England Biolabs Japan Inc.). .. The gene encoding SARS-CoV-2 Mpro with E. coli codon usage was synthesized by Eurofins Genomics (Tokyo, Japan).

    Polymerase Chain Reaction:

    Article Title: Polo regulates Spindly to prevent premature stabilization of kinetochore–microtubule attachments
    Article Snippet: The recombinant proteins were purified using the Ni‐NTA purification system (Qiagen) and eluted with 250 mM Imidazole, 300 mM NaCl, 50 mM NaH 2 PO 4 , pH 8.0. .. For MBP fusion, PCR products harbouring Zwilch or Spindly coding sequences were cloned into EcoRI/XbaI sites of pMal‐c2 expression vector (New England Biolabs). ..

    Article Title: The Membrane-Bound Notch Regulator Mnr Supports Notch Cleavage and Signaling Activity in Drosophila melanogaster
    Article Snippet: .. To this end, the coding sequence was PCR amplified, adding Eco RI and Sal I site, and inserted into pMal-c2 expression vector (NEB, Ipswich, MA, USA) in frame with N-terminal malE to generate pMal-Mnr. ..

    Clone Assay:

    Article Title: Polo regulates Spindly to prevent premature stabilization of kinetochore–microtubule attachments
    Article Snippet: The recombinant proteins were purified using the Ni‐NTA purification system (Qiagen) and eluted with 250 mM Imidazole, 300 mM NaCl, 50 mM NaH 2 PO 4 , pH 8.0. .. For MBP fusion, PCR products harbouring Zwilch or Spindly coding sequences were cloned into EcoRI/XbaI sites of pMal‐c2 expression vector (New England Biolabs). ..

    Synthesized:

    Article Title: Epitope mapping of an anti-diacylglycerol kinase delta monoclonal antibody DdMab-1
    Article Snippet: .. Synthesized DNA (Eurofins Genomics KK, Tokyo, Japan) encoding hDGKδ (accession No. NM_152879 ) plus a C-terminal PA tag (GVAMPGAEDDVV) [ ] was subcloned into the pMAL-c2 expression vector (New England Biolabs Inc., Beverly, MA) using the In-Fusion HD Cloning Kit (Takara Bio, Inc., Shiga, Japan). ..

    Cloning:

    Article Title: Epitope mapping of an anti-diacylglycerol kinase delta monoclonal antibody DdMab-1
    Article Snippet: .. Synthesized DNA (Eurofins Genomics KK, Tokyo, Japan) encoding hDGKδ (accession No. NM_152879 ) plus a C-terminal PA tag (GVAMPGAEDDVV) [ ] was subcloned into the pMAL-c2 expression vector (New England Biolabs Inc., Beverly, MA) using the In-Fusion HD Cloning Kit (Takara Bio, Inc., Shiga, Japan). ..

    Sequencing:

    Article Title: The Membrane-Bound Notch Regulator Mnr Supports Notch Cleavage and Signaling Activity in Drosophila melanogaster
    Article Snippet: .. To this end, the coding sequence was PCR amplified, adding Eco RI and Sal I site, and inserted into pMal-c2 expression vector (NEB, Ipswich, MA, USA) in frame with N-terminal malE to generate pMal-Mnr. ..

    Amplification:

    Article Title: The Membrane-Bound Notch Regulator Mnr Supports Notch Cleavage and Signaling Activity in Drosophila melanogaster
    Article Snippet: .. To this end, the coding sequence was PCR amplified, adding Eco RI and Sal I site, and inserted into pMal-c2 expression vector (NEB, Ipswich, MA, USA) in frame with N-terminal malE to generate pMal-Mnr. ..

    Produced:

    Article Title: Early antibody response against Mycobacterium avium subspecies paratuberculosis antigens in subclinical cattle
    Article Snippet: .. There are 92 total M. paratuberculosis proteins recombinantly produced in E. coli with 89 of these using the pMAL-c2 expression vector (New England Biolabs), and 3 using the pET vector. ..



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